Biodiesel production from microbial lipid obtained by intermittent feeding of municipal sludge and treated crude glycerol

In the present study, municipal secondary sludge and purified glycerol (obtained after acid treatment of crude glycerol) were used together for lipid production using intermittent feeding strategy. Intermittent strategy (sludge SS 30 g/L) resulted in a higher biomass (54.99 concentration (25.35 at 96 h when compared to 35 g/L single or control (45.67 & 19.16 lipid). Moreover, significantly reduced foaming requirement anti-foam during fermentation The energy balance biodiesel from obtained by (30 SS) was energetically favorable. It also revealed yield coefficients that had an important contribution microbial production.